The residues Y65 2.64 , D84 3.32 , K173 5.39 , I240 6.55 , and Y258 7.35 are functional hotspot residues in the 1.1 biased agonist binding site that contribute significantly to the strength of the ...May 30, 2021 · The diploid Y258 host strain genotype was: MATa/MAT pep4-3/pep4-3 his4-580/his4-580 ura3-52/ura3-52 leu2-3/leu2-3 CAN1/can1, MET6/met6::kanMX4. It was propagated in the same media as listed above supplemented with 200 mg/l of geneticin (G418). A mixture of individual host strains was prepared to initiate a starvation experiment as described below. JAX Terminal Badge Required. Badges can be obtained at Jaxport’s Access Control Center. For more information, call (904) 357-3344. Pedestrians permitted on sidewalks and designated walkways. Stop at all stop signs and unmarked crossings. Terminal equipment has the right of way at all times.

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