Blue pippin
Technical note ALTERNATIVE SIZE SELECTION METHODS FOR SMRTBELL ® PREP KIT 3.0 Introduction In Procedure & checklist — Preparing whole genome and metagenome sequencing libraries using SMRTbell prep kit 3.0, size-selection beads are used to remove short DNA fragments and enrich for the long fragments that are most valuable for downstream analysis.Tracks show sRNA mapping (blue) and filtered for 21 nt (red), 22 nt (purple), and 24 nt (green) for lyc and P4042 (top tracks) and WT and dcl2 (bottom tracks). The numbers in brackets show the scale (normalized number of reads) for each track. ... Prof. Jiggins and Ian Warren for providing access to Blue Pippin; SLCU for providing access to ...The Blue Pippin and Pippin Prep allow researchers to easily collect from a mixture of DNA fragment sizes, narrow fragment distributions as well as wide ranges of fragments with minimal effort. Maximum DNA fragment size 50 Kb. Up to 5 samples can be ran at once, with no possibility of cross-contamination. Technical Primary Contact:
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The Blue Pippin is preparative electrophoresis system for targeted size selection of biomolecules. Pippin's disposable gel cassettes are precast with agarose. Each sample lane is physically separate to eliminate the possibility of sample cross-contamination. DNA sizing available with the Blue Pippin is for fragments from 100 bp to 50 Kb. Up ...Blue Pippin Usage. The Blue Pippin is available for user operation for $12/sample after initial training. Alternatively, samples can be dropped off and ran by GSC staff. Please email us at [email protected] for training or to set up a time to drop off samples to be run on the Blue Pippin.This instructional video demonstrates the optical calibration procedure for Pippin Prep and BluePippin automated DNA size selection instruments from Sage Sci...Apr 27, 2018 · Shearing to 10 kb with Covaris G-tubes (5000 x g for 1 min each way) achieves similar results. Alternatively, for longer reads it is recommended not to shear and perform the ligation protocol as recommended, or include a size selection step with the Blue Pippin (Sage Sciences). Sequencing outcomes for all options are given in table below.
Page 3 PN 100-377-300-03 2. Mix contents and spin the tubes briefly in a microcentrifuge. 3. Incubate the tubes at 72°C in a hot-lid thermal cycler for 3 min; slow ramp to 42°C at 0.1°C/sec let sit for 2From the genomic DNA, 20 Kb insert size libraries were prepared and size selected using Blue Pippin™ and sequenced using C3 in combination with P5 polymerase chemistry for 2 SMRT cells per genome with 180 min or longer movie time and stage start.Sage science全自动核酸电泳和片段回收系统Pippin，科技视频免费在线观看，视频简介：Sage science全自动核酸电泳和片段回收系统 Pippin Prep and Blue Pippin 首页 电视剧 电影 云影院 综艺 儿童 动漫 游戏 会员中心 纪录片 体育 知识 直播 小说 漫画 风云榜 新片 公益 VR 随 ...NGS can be roughly divided into the process elements of sample pre-processing, library preparation, sequencing itself and bioinformatics (Fig. 1).Regardless of the underlying principles of the respective sequencing method, all modern sequencing technologies require dedicated sample preparation to yield the sequencing library loaded …
Libraries were prepared with Illumina TruSeq Stranded mRNA kit starting from 600 ng of total RNA, and samples were fragmented and amplified for 15 PCR cycles. Libraries were size selected with Blue Pippin (Sage Science) using 1.5% gel cassettes and 350–550 bp regions isolated.Abstract Root-knot nematodes from the genus Meloidogyne are polyphagous plant endoparasites and agricultural pests of global importance. Here, we report the high-quality genome sequence of Meloidogyne luci population SI-Smartno V13. The resulting genome assembly of M. luci SI-Smartno V13 consists of 327 contigs, with an N50 contig …Sage-Scientific Blue Pippin; Life Technologies E-Gel System; Diagenode Bioruptor Standard Sonicator; Gene Expression. Quantitative PCR (qPCR) Affymetrix GeneChip Workstation; Imaging. Typhoon 9410 GE Healthcare; Biorad Gel Doc EZ; Incubators/Shakers. Labnet Vortemp 56 incubator; Mcconnell Research Mini-grow 384 Incubator; Larger Incubators ….
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Blue Pippin size selection 250bp - 1.5kb Size select samples using the Blue Pippin 1.5% agarose cassette, between 250 bp and 1.5 kb. Smaller size ranges will have lower yields. Cost is per sample. A normal cassette cartridge contains 5 sample lanes with internal standards. Internal $98.00 each External $117.60 each Corporate $162.97 eachThis instructional video from Sage Science guides you through the process of preparing your cassette for use with either the Pippin Prep or BluePippin automa...A subset of the samples were sent to IBIS (n = 96, including 16 samples from each population) for PstI/MspI library preparation following the procedure of Poland et al. with the following modifications: addition of a size selection step using a Blue Pippin 2% agarose cassette with time settings to elute between 45 and 60 min and a plate barcode ...
BLU0001 The BluePippin System includes instrument, monitor, keyboard, and mouse. Dimensions are 7"H X 11"W X 21"D, and weight is 15 lbs. Requires 100-240 VAC.Pippin Prep DNA Size Selection System Selects 100 bpd - 1.5 kb. SageELF Whole Specimen Fractionation System DNA (up to 40 kb) or Proteins (up toward 500 kDa). Pippin Pulses Electrophoresis Power Supply Resolves DNA up to 400kb on an midi gel box. Applications. Transposase Enzyme Linked Long-Read Sequencing ...
arterio Pippin Prep DNA Size Selection System Selects 100 bp – 1.5 kb. SageELF Whole Sample Fractionation System DNA (up to 40 kb) or Proteins (up to 500 kDa). Pippin Pulse ...The selected size for this method was 314-414 bp using the Blue Pippin system. We predicted that there were 117,528 SLAF tags distributed in the genome with 6.19% located in repetitive regions. To further evaluate the efficiency of the restriction enzyme digestion scheme, Arabidopsis thaliana was selected as an example. realistic baby dolls girlbaker wichita Venues really don't come much more picturesque than PIPPIN HILL FARM in the heart of Virginia's wine country. It was an obvious choice for Spencer & Daniel ... tv guide dish network tonight Blue Pippin. Sort by. Blue Pippin . More. Blue Pippin Cassette 3%, 100-250 bp, Internal Marker Q3 . CHF 818.00 . CHF 818.00 . Add to cart More. Blue Pippin Cassette 2%, 100-600 bp, Internal Marker . CHF 818.00 . CHF 818.00 . Add to cart More. Blue Pippin Cassette 2%, 100-600 bp, External Marker . CHF 818.00 . CHF 818.00 ...Size-selection was performed using the Blue Pippin electrophoresis platform (Sage Science), in order to select fragments of 215 bp of length as in [ 70 ], in order to be able to integrate our data ... ba communication studiesou golf teamk+s portal login Run times are as little as 30 minutes. View the Pippin Prep cassette reference chart to explore the DNA size selection options and run times. 3% agarose, 100 - 250 bp. 2% agarose, 100 - 600 bp. 1.5% agarose, 250 bp - 1.5 kb.Then optimized PCR reactions were used to generate double-stranded cDNA, followed by size selection (fragments larger than 4 kb) using a Blue Pippin TM Size-Selection System (Sage Science, MA, United States) as described by PacBio (PN 100-092-800-03). The generated cDNA was then re-amplified using PCR to obtain sufficient cDNA. masters degree in behavioral science selected protocol will be highlighted in blue. g. For the target duration, enter the desired run time (typically 14 to 16 hours). Note that the default value is 2 hours. Consider optimizing the run time based on the ambient lab temperature since the Pippin Pulse Electrophoresis System does not regulate the temperature in the gel chamber.The ColourTex® stainless steel range is unique whereby the colours and tones of the product varies in different lighting conditions. This is known as a 'living' colour when viewed from different angles, also known as metamerism. The ColourTex® process can be combined with the surface finishes of mirror, satin and other polishes, Granex ... sams east wichitahow do you get your teaching licenserock.chalk Blue Pippin and E-gel size selection systems; Agilent Bioanalyzer; Eppendorf epMotion M5073 liquid handling system; GTF Staff are available to answer questions regarding the use of technologies offered by the GTF and provide consultation on experimental design.Product Type. Agarose Gel Cassette. Separation Range. 100 to 600 bp. Quantity. 10 Pack. Provide Content Correction. For size selections between 100bp and 600bp. Sage Science™ BluePippin™ 2% Agarose Gel Cassettes are commonly used for Illumina paired-end library construction, Ion Torrent template prep, and ChIP applications.